Characterization system for therapeutic proteins

Malvern Instruments' Zetasizer Nano particle characterization system has proved highly effective in studies to compare two forms of a therapeutic antibody.

Malvern Instruments' Zetasizer Nano particle characterization system has proved highly effective in studies to compare two forms of a therapeutic antibody - one freshly prepared and the other having undergone a proprietary treatment process.

Such studies are important in understanding the characteristics and behaviour of therapeutic preparations, as well as optimizing their formulation and stability.

The Zetasizer Nano measures particle size, zeta potential and molecular weight in nano-sized particles and molecules in solution.

It is designed specifically to meet the low concentration and sample volume requirements typically associated with pharmaceutical and biomolecular applications.

Using the Zetasizer Nano, treated and untreated antibody preparations were analyzed for hydrodynamic size, molecular weight and protein melting point.

Significant differences in size, size distribution, average molecular weight and melting point between the two indicated that the treatment process used was similar to thermal denaturation.

A melting point transition was seen for the treated sample, but was absent in the fresh preparation.

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